Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-38
... libraries of bacterial lysogens carrying recombinant Agt11 genomes were induced by heating and screened for the subsequent production of foreign antigens . Propagation of recombinant bac- teriophages as lysogens appeared to offer ...
... libraries of bacterial lysogens carrying recombinant Agt11 genomes were induced by heating and screened for the subsequent production of foreign antigens . Propagation of recombinant bac- teriophages as lysogens appeared to offer ...
Page 9-2
... libraries of eukaryotic genomic DNA . Originally devised as banks from which individual bacterial genes could be isolated and analyzed ( Clarke and Carbon 1976 ) , genomic DNA libraries are now increasingly viewed as matrices that can ...
... libraries of eukaryotic genomic DNA . Originally devised as banks from which individual bacterial genes could be isolated and analyzed ( Clarke and Carbon 1976 ) , genomic DNA libraries are now increasingly viewed as matrices that can ...
Page 12-6
... libraries can be constructed in plasmid and bac- teriophage expression vectors : cDNA and genomic DNA libraries . For prokaryotic organisms , whose genomes contain few introns , there is little to be gained by constructing cDNA libraries ...
... libraries can be constructed in plasmid and bac- teriophage expression vectors : cDNA and genomic DNA libraries . For prokaryotic organisms , whose genomes contain few introns , there is little to be gained by constructing cDNA libraries ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector