Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 50
Page 15-8
... linear plasmid is detectable , a new preparation should be made ( see Chapter 1 ) . The major problem with this method is the generation of full - length linear molecules after partial digestion with a restriction enzyme that cleaves ...
... linear plasmid is detectable , a new preparation should be made ( see Chapter 1 ) . The major problem with this method is the generation of full - length linear molecules after partial digestion with a restriction enzyme that cleaves ...
Page 15-9
... linear DNA will be used as an electrophoretic marker ( see h ) . g . After the eight test reactions have incubated for 30 minutes , quickly transfer the tubes to an ice - water bath . Add 5 μl of 50 mм EDTA ( pH 8.0 ) to each tube to ...
... linear DNA will be used as an electrophoretic marker ( see h ) . g . After the eight test reactions have incubated for 30 minutes , quickly transfer the tubes to an ice - water bath . Add 5 μl of 50 mм EDTA ( pH 8.0 ) to each tube to ...
Page 15-89
... linear DNA and considerably faster than relaxed circular DNA . d . Examine the gel by ultraviolet illumination , and determine the condi- tions that give the maximum yield of full - length linear molecules . Usually not more than 33 ...
... linear DNA and considerably faster than relaxed circular DNA . d . Examine the gel by ultraviolet illumination , and determine the condi- tions that give the maximum yield of full - length linear molecules . Usually not more than 33 ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
88 other sections not shown
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector