Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 15-7
... linkers are then ligated directly to these blunt ends . An alternative strategy is to use linkers that carry protruding termini complementary to the termini generated by the restriction enzyme ( Barany 1985a , b ) . Hexa- meric linkers ...
... linkers are then ligated directly to these blunt ends . An alternative strategy is to use linkers that carry protruding termini complementary to the termini generated by the restriction enzyme ( Barany 1985a , b ) . Hexa- meric linkers ...
Page 15-10
... linker ( in nucleotides ) , y = length of linear plasmid DNA ( in nucleotides ) , and z = desired molar ratio of linkers to target DNA . Usually , a 20- to 30 - fold molar excess of linkers is used to ensure that several copies of the ...
... linker ( in nucleotides ) , y = length of linear plasmid DNA ( in nucleotides ) , and z = desired molar ratio of linkers to target DNA . Usually , a 20- to 30 - fold molar excess of linkers is used to ensure that several copies of the ...
Page 15-86
... linkers must be used in place of single - stranded linkers . ) • The restriction site that is to be inserted as part of the hexameric linker should not occur at any other place in the target DNA or the plasmid vector . • The choice of ...
... linkers must be used in place of single - stranded linkers . ) • The restriction site that is to be inserted as part of the hexameric linker should not occur at any other place in the target DNA or the plasmid vector . • The choice of ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml