Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 82
Page 8-4
... mRNA from which it is derived , it is important to check the integrity of the preparation of mRNA before it is used as the template for synthesis of the first strand of cDNA . The following tests are commonly used : The ability of the mRNA ...
... mRNA from which it is derived , it is important to check the integrity of the preparation of mRNA before it is used as the template for synthesis of the first strand of cDNA . The following tests are commonly used : The ability of the mRNA ...
Page 8-6
... mRNA is required before double - stranded cDNA is synthesized and cloned . The desired cDNA clones can easily be ... mRNAs 8 Low-abundance mRNAs 8 Methods of Enrichment 8.
... mRNA is required before double - stranded cDNA is synthesized and cloned . The desired cDNA clones can easily be ... mRNAs 8 Low-abundance mRNAs 8 Methods of Enrichment 8.
Page 8-48
... mRNA is depleted of sequences that are present in a second type of mRNA by subtractive hybridization ( Timberlake 1980 ; Zimmerman et al . 1980 ) . Typically , the cDNA is hybridized two or three times in succession to a 20 - fold ...
... mRNA is depleted of sequences that are present in a second type of mRNA by subtractive hybridization ( Timberlake 1980 ; Zimmerman et al . 1980 ) . Typically , the cDNA is hybridized two or three times in succession to a 20 - fold ...
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml