Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-44
... membrane about 1 mm larger than the gel in both dimensions . Use gloves and blunt- ended forceps ( e.g. , Millipore forceps ) to handle the membrane . b . Float the membrane on the surface of a dish of deionized water until it wets ...
... membrane about 1 mm larger than the gel in both dimensions . Use gloves and blunt- ended forceps ( e.g. , Millipore forceps ) to handle the membrane . b . Float the membrane on the surface of a dish of deionized water until it wets ...
Page 9-45
... membrane has been exposed to high concentrations of alkali for extended periods of time . This problem can often be ... membrane as follows : a . Using a fresh scalpel or a paper cutter , cut a piece of membrane about 1 mm larger than ...
... membrane has been exposed to high concentrations of alkali for extended periods of time . This problem can often be ... membrane as follows : a . Using a fresh scalpel or a paper cutter , cut a piece of membrane about 1 mm larger than ...
Page 9-46
... membrane from the neutralizing solution and allow excess fluid to drain away . Place the membrane flat on a paper towel to dry for at least 30 minutes at room temperature . 7. To fix the DNA to the membrane either • place the dried ...
... membrane from the neutralizing solution and allow excess fluid to drain away . Place the membrane flat on a paper towel to dry for at least 30 minutes at room temperature . 7. To fix the DNA to the membrane either • place the dried ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml