Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 58
Page 13-26
... microfuge tube . Store this tube on ice . Between bursts , centrifuge the original mi- crofuge tube for 2 seconds to deposit the DNA solution at the bottom of the tube , and add fresh ice to the sonicator horn to maintain the ...
... microfuge tube . Store this tube on ice . Between bursts , centrifuge the original mi- crofuge tube for 2 seconds to deposit the DNA solution at the bottom of the tube , and add fresh ice to the sonicator horn to maintain the ...
Page 13-61
... microfuge tube , and then add 2 μl of single - stranded DNA template . Close the top of the tube , and incubate it for 30 minutes at 55 ° C . The concentration of single - stranded DNA in small - scale preparations of bac- teriophage ...
... microfuge tube , and then add 2 μl of single - stranded DNA template . Close the top of the tube , and incubate it for 30 minutes at 55 ° C . The concentration of single - stranded DNA in small - scale preparations of bac- teriophage ...
Page 14-14
... microfuge tubes containing reagents used in polymerase chain reactions , centrifuge them briefly ( 10 seconds ) in the microfuge locat- ed in the laminar flow hood . This deposits the fluid in the base of the tube and reduces the ...
... microfuge tubes containing reagents used in polymerase chain reactions , centrifuge them briefly ( 10 seconds ) in the microfuge locat- ed in the laminar flow hood . This deposits the fluid in the base of the tube and reduces the ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml