Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 80
Page 13-40
... mixture . To the remainder of the DNA solution , add 150 units of exonuclease III per picomole of recessed 3 ' termini . Vortex briefly to mix the contents of the tube , and immediately return the tube to the 37 ° C water bath . 5. At ...
... mixture . To the remainder of the DNA solution , add 150 units of exonuclease III per picomole of recessed 3 ' termini . Vortex briefly to mix the contents of the tube , and immediately return the tube to the 37 ° C water bath . 5. At ...
Page 13-62
... mixture at room temperature . 6. At the end of the incubation ( step 2 ) , remove the primer - template mixture from the 55 ° C incubator . Centrifuge the tube briefly in a microfuge to return any condensation to the bottom of the tube ...
... mixture at room temperature . 6. At the end of the incubation ( step 2 ) , remove the primer - template mixture from the 55 ° C incubator . Centrifuge the tube briefly in a microfuge to return any condensation to the bottom of the tube ...
Page 15-65
... mixture to the reaction mixture containing single - stranded DNA and annealed oligonucleotides ( step 3 ) . Incubate the final reaction mixture for 6-15 hours at 16 ° C . 6. Transfect competent E. coli of an appropriate host strain ...
... mixture to the reaction mixture containing single - stranded DNA and annealed oligonucleotides ( step 3 ) . Incubate the final reaction mixture for 6-15 hours at 16 ° C . 6. Transfect competent E. coli of an appropriate host strain ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml