Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-23
... molecules . The reaction often proceeds asynchronously , so that different termini of DNA molecules carry homopolymeric tails of very different lengths . This prob- lem can be alleviated , but not completely solved , by using plasmids ...
... molecules . The reaction often proceeds asynchronously , so that different termini of DNA molecules carry homopolymeric tails of very different lengths . This prob- lem can be alleviated , but not completely solved , by using plasmids ...
Page 8-24
... molecules with cohesive termini . These molecules are then purified and ligated to a large molar excess of the appropriate dephosphorylated vector . • The use of synthetic adapters rather than linkers . Adapters are short , double ...
... molecules with cohesive termini . These molecules are then purified and ligated to a large molar excess of the appropriate dephosphorylated vector . • The use of synthetic adapters rather than linkers . Adapters are short , double ...
Page 15-27
... molecules . Few of the molecules are cleaved more than once by the enzyme . Digestion with a restriction enzyme that cleaves at one end of the target sequence therefore generates a mixed population that contains full - length linear ...
... molecules . Few of the molecules are cleaved more than once by the enzyme . Digestion with a restriction enzyme that cleaves at one end of the target sequence therefore generates a mixed population that contains full - length linear ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml