Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 51
Page 15-33
... mutants . Although the original method for con- structing such mutants was later streamlined ( Haltiner et al . 1985 ) , the problem of obtaining a sufficient number of well - characterized 5 ' and 3 ' deletions remains a serious ...
... mutants . Although the original method for con- structing such mutants was later streamlined ( Haltiner et al . 1985 ) , the problem of obtaining a sufficient number of well - characterized 5 ' and 3 ' deletions remains a serious ...
Page 15-59
... Mutants After the primer - extension reaction is complete , the resulting mixture of double - stranded heteroduplex DNA is then transfected directly into an appro- priate bacterial host . Most of the transfected cells release ...
... Mutants After the primer - extension reaction is complete , the resulting mixture of double - stranded heteroduplex DNA is then transfected directly into an appro- priate bacterial host . Most of the transfected cells release ...
Page 15-96
... mutants . However , the mutants cannot generally be distinguished from the original wild - type DNA by the standard method of screening by hybridization . In most cases , the mutagenic oligonucleotides are so long that there is no ...
... mutants . However , the mutants cannot generally be distinguished from the original wild - type DNA by the standard method of screening by hybridization . In most cases , the mutagenic oligonucleotides are so long that there is no ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector