Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 60
Page 9-38
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Transfer of DNA to Nitrocellulose Filters CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 1. After electrophoresis , transfer the gel to a glass baking dish and trim away any unused ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Transfer of DNA to Nitrocellulose Filters CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 1. After electrophoresis , transfer the gel to a glass baking dish and trim away any unused ...
Page 9-39
... nitrocellulose filter to match the corner cut from the gel . The rate at which different batches of nitrocellulose filters wet varies enormously . If the filter is not saturated after floating for several minutes on water , it should be ...
... nitrocellulose filter to match the corner cut from the gel . The rate at which different batches of nitrocellulose filters wet varies enormously . If the filter is not saturated after floating for several minutes on water , it should be ...
Page 9-41
... NITROCELLULOSE FILTERS DNA can be transferred simultaneously from a single agarose gel to two nitrocellulose filters as described by Smith and Summers ( 1980 ) ( see Figure 9.4 ) . 1. After electrophoresis , prepare the gel for transfer ...
... NITROCELLULOSE FILTERS DNA can be transferred simultaneously from a single agarose gel to two nitrocellulose filters as described by Smith and Summers ( 1980 ) ( see Figure 9.4 ) . 1. After electrophoresis , prepare the gel for transfer ...
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml