Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-7
... nucleotides would therefore be expected to be represented only once in the genome . However , the distribution of nucleotides in the coding sequences of mammalian genomes is nonrandom ( Lathe 1985 ) , and it is therefore advisable to ...
... nucleotides would therefore be expected to be represented only once in the genome . However , the distribution of nucleotides in the coding sequences of mammalian genomes is nonrandom ( Lathe 1985 ) , and it is therefore advisable to ...
Page 15-54
... nucleotides ) the problem region . Screening for the desired mutants is then carried out with a much smaller oligonucleotide ( 17-19 nucleotides ) that carries the potentially mismatched base ( s ) in the central position . The sequence ...
... nucleotides ) the problem region . Screening for the desired mutants is then carried out with a much smaller oligonucleotide ( 17-19 nucleotides ) that carries the potentially mismatched base ( s ) in the central position . The sequence ...
Page 15-55
... nucleotides are required at the 3 ' terminus of the mutagenic oligonu- cleotide . • The difference in thermal ... nucleotides in length , with the mismatch centrally located . 2. Oligonucleotides used to create deletions or insertions or ...
... nucleotides are required at the 3 ' terminus of the mutagenic oligonu- cleotide . • The difference in thermal ... nucleotides in length , with the mismatch centrally located . 2. Oligonucleotides used to create deletions or insertions or ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml