Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-7
... obtained from single - stranded DNA templates , which are usually isolated from recombinant bacteriophage M13 particles . When care is taken to optimize the ratio of single - stranded template to primer , it is possible to obtain ...
... obtained from single - stranded DNA templates , which are usually isolated from recombinant bacteriophage M13 particles . When care is taken to optimize the ratio of single - stranded template to primer , it is possible to obtain ...
Page 13-15
... obtained in a systematic fashion . For example , the entire sequence of the target DNA might be obtained by sequencing a nested set of deletion mutants that begin at a common point ( usually at one end of the target DNA ) and penetrate ...
... obtained in a systematic fashion . For example , the entire sequence of the target DNA might be obtained by sequencing a nested set of deletion mutants that begin at a common point ( usually at one end of the target DNA ) and penetrate ...
Page 13-95
... obtained from DNA sequenced by the Sanger method , which uses [ 35S ] dNTPs . This lack of resolution limits the amount of reliable sequence that can be obtained from a single gel to approximately 200-250 nucleotides . Problems Commonly ...
... obtained from DNA sequenced by the Sanger method , which uses [ 35S ] dNTPs . This lack of resolution limits the amount of reliable sequence that can be obtained from a single gel to approximately 200-250 nucleotides . Problems Commonly ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml