Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-21
... Oligonucleotides Oligonucleotides are usually supplied as a lyophilized powder . 1. In a sterile microfuge tube , dissolve the crude oligonucleotide ( usually 1-10 nmoles ) in 1 ml of sterile , filtered H2O ( Milli - Q or equivalent ) ...
... Oligonucleotides Oligonucleotides are usually supplied as a lyophilized powder . 1. In a sterile microfuge tube , dissolve the crude oligonucleotide ( usually 1-10 nmoles ) in 1 ml of sterile , filtered H2O ( Milli - Q or equivalent ) ...
Page 11-30
... oligonucleotide present in the original solution . Amounts of oligonucleotides are often described in OD units . One OD corresponds to the amount of oligonucleotide in a 1 - ml volume that results in an optical density of 1 in a 1 - cm ...
... oligonucleotide present in the original solution . Amounts of oligonucleotides are often described in OD units . One OD corresponds to the amount of oligonucleotide in a 1 - ml volume that results in an optical density of 1 in a 1 - cm ...
Page 15-80
... oligonucleotide by polyacrylamide gel electro- phoresis . • Test the mutagenic oligonucleotide for its ability to prime DNA synthe- sis at the appropriate location on the single - stranded template DNA ( Zoller and Smith 1987 ) . Use ...
... oligonucleotide by polyacrylamide gel electro- phoresis . • Test the mutagenic oligonucleotide for its ability to prime DNA synthe- sis at the appropriate location on the single - stranded template DNA ( Zoller and Smith 1987 ) . Use ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml