Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 32
Page 9-29
... pellet of nucleic acid with 70 % ethanol and recentrifuge for 2 minutes . Remove the ethanol by careful aspiration , and allow the pellet to dry at room temperature . Redissolve the pellet in TE ( pH 7.6 ) at a concentration of 1 μg ...
... pellet of nucleic acid with 70 % ethanol and recentrifuge for 2 minutes . Remove the ethanol by careful aspiration , and allow the pellet to dry at room temperature . Redissolve the pellet in TE ( pH 7.6 ) at a concentration of 1 μg ...
Page 13-83
... pellet after each wash and check the amount of radioac- tivity in both the pellet and the wash with a hand - held minimonitor before the ethanol is discarded . 4. After the final wash , carefully remove the ethanol by aspiration , and ...
... pellet after each wash and check the amount of radioac- tivity in both the pellet and the wash with a hand - held minimonitor before the ethanol is discarded . 4. After the final wash , carefully remove the ethanol by aspiration , and ...
Page 13-98
... pellet , and poor vacuum during the lyophilization steps are common causes of this problem . Hydrazine has undergone oxidation . Residual salt from ethanol precipitation of end - labeled DNA is carried over into the sequencing reactions ...
... pellet , and poor vacuum during the lyophilization steps are common causes of this problem . Hydrazine has undergone oxidation . Residual salt from ethanol precipitation of end - labeled DNA is carried over into the sequencing reactions ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml