Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-73
... plaques on strains of E. coli such as Y1090hsdR in the presence of the inducer IPTG and the chromogenic substrate X - gal ; recombinant plaques are colorless . Nonrecombinant bacteriophages do not present a problem when bac- teriophages ...
... plaques on strains of E. coli such as Y1090hsdR in the presence of the inducer IPTG and the chromogenic substrate X - gal ; recombinant plaques are colorless . Nonrecombinant bacteriophages do not present a problem when bac- teriophages ...
Page 8-75
... plaques . Ligation A should yield no recombinant plaques , whereas ligations B , C , and D should yield increasing numbers of recombinant plaques . From the number of recombinant plaques , calculate the efficiency of cloning of cDNA ( ...
... plaques . Ligation A should yield no recombinant plaques , whereas ligations B , C , and D should yield increasing numbers of recombinant plaques . From the number of recombinant plaques , calculate the efficiency of cloning of cDNA ( ...
Page 12-16
... plaques is low . The color produced by the chromogenic reaction is most intense at the expanding edge of the plaque . Well - isolated immunopositive plaques therefore display a characteristic ringlike pattern . When plaques crowd ...
... plaques is low . The color produced by the chromogenic reaction is most intense at the expanding edge of the plaque . Well - isolated immunopositive plaques therefore display a characteristic ringlike pattern . When plaques crowd ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector