Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 78
Page vi
... Plasmid DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF PLASMID DNA 1.33 Amplification of Plasmids in Rich Medium 1.33 Harvesting and Lysis of Bacteria 1.34 HARVESTING 1.34 ...
... Plasmid DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF PLASMID DNA 1.33 Amplification of Plasmids in Rich Medium 1.33 Harvesting and Lysis of Bacteria 1.34 HARVESTING 1.34 ...
Page 15-8
... plasmid DNA . • An aliquot of the preparation of recombinant plasmid DNA that is to be used as a substrate for mutagenesis should be analyzed by agarose gel electrophoresis . It should consist predominantly of superhelical molecules ...
... plasmid DNA . • An aliquot of the preparation of recombinant plasmid DNA that is to be used as a substrate for mutagenesis should be analyzed by agarose gel electrophoresis . It should consist predominantly of superhelical molecules ...
Page 15-9
... DNA mixture to the second tube . Mix quickly , and replace the tube on ice . d . Using another fresh pipette tip ... plasmid DNA with an excess ( 5 units ) of a restriction enzyme that cleaves the DNA only once . The resulting linear DNA ...
... DNA mixture to the second tube . Mix quickly , and replace the tube on ice . d . Using another fresh pipette tip ... plasmid DNA with an excess ( 5 units ) of a restriction enzyme that cleaves the DNA only once . The resulting linear DNA ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml