Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-24
... plates . Rinse the plates with ethanol and set them aside to dry . The plates must be free of grease spots to prevent air bubbles from forming in the gel . Treat one surface of each plate with a silicone solution . This prevents the gel ...
... plates . Rinse the plates with ethanol and set them aside to dry . The plates must be free of grease spots to prevent air bubbles from forming in the gel . Treat one surface of each plate with a silicone solution . This prevents the gel ...
Page 13-49
... plate , lay the plate on a pad of paper in a chemical hood , and pour a small quantity of siliconizing fluid ( e.g. , Sigmacote ) onto it . Wipe the fluid over the surface of the plate with a pad of Kimwipes , and then rinse the plate ...
... plate , lay the plate on a pad of paper in a chemical hood , and pour a small quantity of siliconizing fluid ( e.g. , Sigmacote ) onto it . Wipe the fluid over the surface of the plate with a pad of Kimwipes , and then rinse the plate ...
Page 13-56
... plate uppermost . Remove any remaining pieces of electrical tape . Using the end of a metal spatula , slowly pry apart the plates of the mold . With luck , the gel will remain attached to the lower plate . If it does not , place the plate ...
... plate uppermost . Remove any remaining pieces of electrical tape . Using the end of a metal spatula , slowly pry apart the plates of the mold . With luck , the gel will remain attached to the lower plate . If it does not , place the plate ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml