Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 16
Page 10-20
... pmoles ( 50 μCi ) of a radiolabeled dNTP ( sp . act . 3000 Ci / mmole ) , and 2 pmoles of template ( ~ 1 μg ) , an average of only 28 nucleotides will be added to each primer molecule before the synthesis is brought to a halt by a lack ...
... pmoles ( 50 μCi ) of a radiolabeled dNTP ( sp . act . 3000 Ci / mmole ) , and 2 pmoles of template ( ~ 1 μg ) , an average of only 28 nucleotides will be added to each primer molecule before the synthesis is brought to a halt by a lack ...
Page 10-23
... pmoles of dATP ( 16 pmoles radiolabeled and 40 pmoles unlabeled ) , a total of 28 pmoles of dATP has been incorporated into DNA . Assuming that each of the four dNTPs is equally represented in the product , a total of 4 × 28 = 112 pmoles ...
... pmoles of dATP ( 16 pmoles radiolabeled and 40 pmoles unlabeled ) , a total of 28 pmoles of dATP has been incorporated into DNA . Assuming that each of the four dNTPs is equally represented in the product , a total of 4 × 28 = 112 pmoles ...
Page 14-28
... pmoles ) and different amounts of the other ( 0.25 pmole , 1 pmole , and 4 pmoles ) . The amount of single - stranded product in each of the reactions can be estimated at the end of the amplification by agarose gel electrophoresis ...
... pmoles ) and different amounts of the other ( 0.25 pmole , 1 pmole , and 4 pmoles ) . The amount of single - stranded product in each of the reactions can be estimated at the end of the amplification by agarose gel electrophoresis ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml