Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 18
Page 8-44
... polycloning region that may be used for directional cloning of cDNA molecules , including an EcoRI cleavage site analogous to that of Agt11 . • The presence of promoters from bacteriophages T3 and T7 that flank the polycloning region ...
... polycloning region that may be used for directional cloning of cDNA molecules , including an EcoRI cleavage site analogous to that of Agt11 . • The presence of promoters from bacteriophages T3 and T7 that flank the polycloning region ...
Page 10-29
... polycloning site . The range of cloning sites in these vectors is now so large that foreign DNA sequences can usually be inserted by directional cloning no further than 30-40 nucleotides downstream from the position at which RNA ...
... polycloning site . The range of cloning sites in these vectors is now so large that foreign DNA sequences can usually be inserted by directional cloning no further than 30-40 nucleotides downstream from the position at which RNA ...
Page 13-80
... Polycloning Site Hindill Sall BamHI Smal Pstl Xbal Th111 Tth 1111 BstEll EcoRI Sacl In PSP64CS , the Hindill site in the polycloning site is downstream from the SP6 promoter . In PSP65CS , the EcoRI site in the polycloning site is ...
... Polycloning Site Hindill Sall BamHI Smal Pstl Xbal Th111 Tth 1111 BstEll EcoRI Sacl In PSP64CS , the Hindill site in the polycloning site is downstream from the SP6 promoter . In PSP65CS , the EcoRI site in the polycloning site is ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml