Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 36
Page 14-14
... polymerase chain reactions have been set up . Components of the Polymerase Chain Reaction OLIGONUCLEOTIDES Oligonucleotides used for 14.14 In Vitro Amplification of DNA by the Polymerase Chain Reaction AMPLIFICATION METHODS 14 14 14.
... polymerase chain reactions have been set up . Components of the Polymerase Chain Reaction OLIGONUCLEOTIDES Oligonucleotides used for 14.14 In Vitro Amplification of DNA by the Polymerase Chain Reaction AMPLIFICATION METHODS 14 14 14.
Page 14-15
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Components of the Polymerase Chain Reaction OLIGONUCLEOTIDES Oligonucleotides used for priming the ... Polymerase Chain Reaction 14 15 15 BUFFERS USED FOR POLYMERASE CHAIN REACTIONS 14 15.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Components of the Polymerase Chain Reaction OLIGONUCLEOTIDES Oligonucleotides used for priming the ... Polymerase Chain Reaction 14 15 15 BUFFERS USED FOR POLYMERASE CHAIN REACTIONS 14 15.
Page 14-16
... reaction , and any change in their concentration affects the concentration of available Mg ** . We recommend setting up a set of ... Chain Reaction Taq DNA POLYMERASE 14 16 DEOXYRIBONUCLEOSIDE TRIPHOSPHATES 14 16 TARGET SEQUENCES 14 16.
... reaction , and any change in their concentration affects the concentration of available Mg ** . We recommend setting up a set of ... Chain Reaction Taq DNA POLYMERASE 14 16 DEOXYRIBONUCLEOSIDE TRIPHOSPHATES 14 16 TARGET SEQUENCES 14 16.
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
88 other sections not shown
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector