Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 46
Page 11-9
... position of codon ( 5 ' terminus ) C 1 2 3 4 6 Met Phe Ile Val Leu Trp Tyr Pro Ser His Thr Arg Gln Ala Asn Gly Lys Asp Glu Cys 2nd position of codon U с A G บ บ บ Phe UCU Ser UAU Tyr UGU Cys UUC Phe UCC Ser UAC Tyr UGC Cys U UUA Leu UCA ...
... position of codon ( 5 ' terminus ) C 1 2 3 4 6 Met Phe Ile Val Leu Trp Tyr Pro Ser His Thr Arg Gln Ala Asn Gly Lys Asp Glu Cys 2nd position of codon U с A G บ บ บ Phe UCU Ser UAU Tyr UGU Cys UUC Phe UCC Ser UAC Tyr UGC Cys U UUA Leu UCA ...
Page 11-11
... position ( " guessmers " ) . In many cases , only a single oligonucleotide 30–70 nucleotides in length is synthesized that contains the combination of codons most likely to match the authentic gene . • Hybridization with ...
... position ( " guessmers " ) . In many cases , only a single oligonucleotide 30–70 nucleotides in length is synthesized that contains the combination of codons most likely to match the authentic gene . • Hybridization with ...
Page 11-17
... position of codons that code for the same amino acid and differ by A / G in the third position ( e.g. , Glu : GAA / GAG ) . However , the use of inosine in codons that differ by T / C in their third position is of less value ...
... position of codons that code for the same amino acid and differ by A / G in the third position ( e.g. , Glu : GAA / GAG ) . However , the use of inosine in codons that differ by T / C in their third position is of less value ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml