Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-5
... possible sequences that can code for a given tract of amino acids have been used as hybridization probes to isolate both cDNA clones ( see , e.g. , Goeddel et al . 1980 ; Sood et al . 1981 ; Suggs et al . 1981a ; Wallace et al . 1981 ) ...
... possible sequences that can code for a given tract of amino acids have been used as hybridization probes to isolate both cDNA clones ( see , e.g. , Goeddel et al . 1980 ; Sood et al . 1981 ; Suggs et al . 1981a ; Wallace et al . 1981 ) ...
Page 11-10
... possible to avoid incorporating the two incorrect codons TTT and TTC ( both of which code for Phe ) into the oligonucleotide . 3. If enough amino acid sequence is available , it may be possible to design nonoverlapping or partially ...
... possible to avoid incorporating the two incorrect codons TTT and TTC ( both of which code for Phe ) into the oligonucleotide . 3. If enough amino acid sequence is available , it may be possible to design nonoverlapping or partially ...
Page 11-15
... possible , and often desirable , to synthesize a small pool of 2-8 guessmers that contains all possible choices at certain positions . It is best to carry out this kind of limited substitution when an amino acid whose codon is highly ...
... possible , and often desirable , to synthesize a small pool of 2-8 guessmers that contains all possible choices at certain positions . It is best to carry out this kind of limited substitution when an amino acid whose codon is highly ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml