Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 68
Page xxxii
... Precipitation with Ethanol or Isopropanol E.10 PRECIPITATION OF DNA IN MICROFUGE TUBES E.12 PRECIPITATION OF RNA WITH ETHANOL E.15 PRECIPITATION OF LARGE RNAS WITH LITHIUM CHLORIDE E.15 CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH ...
... Precipitation with Ethanol or Isopropanol E.10 PRECIPITATION OF DNA IN MICROFUGE TUBES E.12 PRECIPITATION OF RNA WITH ETHANOL E.15 PRECIPITATION OF LARGE RNAS WITH LITHIUM CHLORIDE E.15 CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH ...
Page 11-33
... precipitation with ethanol if the oligonucleotide is more than 18 nucleotides in length or by precipitation with cetylpyridinium bromide if the oligonucleotide is shorter . If the oligonu- cleotide is to be used in an enzymatic reaction ...
... precipitation with ethanol if the oligonucleotide is more than 18 nucleotides in length or by precipitation with cetylpyridinium bromide if the oligonucleotide is shorter . If the oligonu- cleotide is to be used in an enzymatic reaction ...
Page 13-98
... precipitation e . Poor suppression of Ts in the C ladder 2. Ts are weak in the C + T ladder . 3. Bands are present in C and / or C + T ladders at all guanine positions , not just those where guanine follows a pyrimi- dine in the ...
... precipitation e . Poor suppression of Ts in the C ladder 2. Ts are weak in the C + T ladder . 3. Bands are present in C and / or C + T ladders at all guanine positions , not just those where guanine follows a pyrimi- dine in the ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector