Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 83
Page 9-7
... prepare the genomic DNA for cloning and to recover fragments from specific recombinants . • The ease with which arms can be prepared . A few bacteriophage A vectors have been designed to allow recombinants to be selected by growth in ...
... prepare the genomic DNA for cloning and to recover fragments from specific recombinants . • The ease with which arms can be prepared . A few bacteriophage A vectors have been designed to allow recombinants to be selected by growth in ...
Page 9-12
... prepared simply by cleavage of the bacteriophage λ DNA with the appropriate restriction enzyme . Recombi- nants are selectively propagated on recBC strains of E. coli that are lysogenic for bacteriophage P2 . Nonrecombinants formed by ...
... prepared simply by cleavage of the bacteriophage λ DNA with the appropriate restriction enzyme . Recombi- nants are selectively propagated on recBC strains of E. coli that are lysogenic for bacteriophage P2 . Nonrecombinants formed by ...
Page 10-18
... prepared in vitro from DNA templates by ( 1 ) synthesis of radiolabeled DNA that is complementary to sequences ... Preparation of Radiolabeled DNA and RNA Probes Preparation of Single-stranded Probes 10 18.
... prepared in vitro from DNA templates by ( 1 ) synthesis of radiolabeled DNA that is complementary to sequences ... Preparation of Radiolabeled DNA and RNA Probes Preparation of Single-stranded Probes 10 18.
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector