Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 80
Page 8-44
... presence of six unique restriction sites within the polycloning region that may be used for directional cloning of cDNA molecules , including an EcoRI cleavage site analogous to that of Agt11 . • The presence of promoters from ...
... presence of six unique restriction sites within the polycloning region that may be used for directional cloning of cDNA molecules , including an EcoRI cleavage site analogous to that of Agt11 . • The presence of promoters from ...
Page 9-8
... presence of amber mutations in the arms . Amber mutations are carried in essential genes in many currently used vectors ( or their derivatives ) . This allows genomic DNA libraries to be screened for sequences of interest by the elegant ...
... presence of amber mutations in the arms . Amber mutations are carried in essential genes in many currently used vectors ( or their derivatives ) . This allows genomic DNA libraries to be screened for sequences of interest by the elegant ...
Page 15-72
... presence of the desired mutation and to confirm that no adventitious mutations have arisen . • Recover the mutated fragment , reclone it into an appropriate vector , and confirm the presence of the mutation by Southern hybridization as ...
... presence of the desired mutation and to confirm that no adventitious mutations have arisen . • Recover the mutated fragment , reclone it into an appropriate vector , and confirm the presence of the mutation by Southern hybridization as ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml