Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxvii
... PROMOTERS 17.11 The Bacteriophage λ p1 Promoter 17.11 The trp - lac Promoter 17.13 The Bacteriophage T7 Promoter 17.15 EXPRESSION OF EUKARYOTIC GENES : PROMOTERS AND RIBOSOME- BINDING SITES 17.17 Preparation of a DNA Fragment Containing ...
... PROMOTERS 17.11 The Bacteriophage λ p1 Promoter 17.11 The trp - lac Promoter 17.13 The Bacteriophage T7 Promoter 17.15 EXPRESSION OF EUKARYOTIC GENES : PROMOTERS AND RIBOSOME- BINDING SITES 17.17 Preparation of a DNA Fragment Containing ...
Page 10-5
A Laboratory Manual Joseph Sambrook, Tom Maniatis. T7 promoter T7 promoter www FIGURE 10.2 polycloning site SP6 promoter Clone the target sequences into a plasmid that carries promoters for bacteriophage- encoded DNA - dependent RNA ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. T7 promoter T7 promoter www FIGURE 10.2 polycloning site SP6 promoter Clone the target sequences into a plasmid that carries promoters for bacteriophage- encoded DNA - dependent RNA ...
Page 10-29
... promoter . It is also important to consider the disposition of restriction sites within the template DNA and downstream from it . The 5 ' terminus of the transcript is fixed by the bacteriophage promoter , but the 3 ' terminus is ...
... promoter . It is also important to consider the disposition of restriction sites within the template DNA and downstream from it . The 5 ' terminus of the transcript is fixed by the bacteriophage promoter , but the 3 ' terminus is ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml