Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 84
Page 8-62
... purified from particles of avian ret- roviruses : poly ( A ) RNA ( 1 mg / ml ) 10.0 με oligo ( dT ) 12-18 ( 1 mg / ml ) 10.0 μl 1 м Tris Cl ( pH 8.3 ) at 42 ° C 2.5 με 1 M KCl 7.0 μl 250 mM MgCl2 2.0 με mixture of dNTPs ( 5 mm each ) ...
... purified from particles of avian ret- roviruses : poly ( A ) RNA ( 1 mg / ml ) 10.0 με oligo ( dT ) 12-18 ( 1 mg / ml ) 10.0 μl 1 м Tris Cl ( pH 8.3 ) at 42 ° C 2.5 με 1 M KCl 7.0 μl 250 mM MgCl2 2.0 με mixture of dNTPs ( 5 mm each ) ...
Page 12-13
... purified by affinity chromatography on columns of the immobilized antigen . Note that affinity purification must be carried out with an independent preparation of antigen that is free of the contaminants present in the material used for ...
... purified by affinity chromatography on columns of the immobilized antigen . Note that affinity purification must be carried out with an independent preparation of antigen that is free of the contaminants present in the material used for ...
Page 13-24
... purified target DNA . Separate the target DNA from the vector by preparative gel electrophoresis , and recover the target DNA from the gel by one of the methods described in Chapter 6. Redissolve the purified DNA in 15 μl of TE ( pH 7.6 ) ...
... purified target DNA . Separate the target DNA from the vector by preparative gel electrophoresis , and recover the target DNA from the gel by one of the methods described in Chapter 6. Redissolve the purified DNA in 15 μl of TE ( pH 7.6 ) ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml