Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 86
Page 8-60
... reaction described above . To analyze the material produced in the reaction , it is necessary to carry out a small - scale parallel reaction : a . After all components of the large - scale reaction have been mixed at 4 ° C , place 2.5 ...
... reaction described above . To analyze the material produced in the reaction , it is necessary to carry out a small - scale parallel reaction : a . After all components of the large - scale reaction have been mixed at 4 ° C , place 2.5 ...
Page 11-31
... reaction is carried out efficiently , the specific activity of such probes can be as high as the specific activity of the [ y - 32P ] ATP itself . The reaction described below is designed to label 10 pmoles of an oligonucleotide to high ...
... reaction is carried out efficiently , the specific activity of such probes can be as high as the specific activity of the [ y - 32P ] ATP itself . The reaction described below is designed to label 10 pmoles of an oligonucleotide to high ...
Page 13-43
... reactions using Sequenases , a preliminary step — an incorporation reaction - is included to ensure efficient incorporation of radioactive precursor in the newly synthesized DNA . Until a few years ago , chain - extension / chain ...
... reactions using Sequenases , a preliminary step — an incorporation reaction - is included to ensure efficient incorporation of radioactive precursor in the newly synthesized DNA . Until a few years ago , chain - extension / chain ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector