Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 76
Page 15-32
... regions in DNA usually begins with the analysis of nested sets of deletions that penetrate into the region of interest from the upstream and downstream directions . Such mutants , which can be generated by techniques described elsewhere ...
... regions in DNA usually begins with the analysis of nested sets of deletions that penetrate into the region of interest from the upstream and downstream directions . Such mutants , which can be generated by techniques described elsewhere ...
Page 15-44
... region . 3. From the numbers of colonies obtained and the proportion of clones with a BglII linker in the target region , determine which pilot ligation reaction ( s ) yielded the largest number ( s ) of Kan ' Amp ' colonies and ...
... region . 3. From the numbers of colonies obtained and the proportion of clones with a BglII linker in the target region , determine which pilot ligation reaction ( s ) yielded the largest number ( s ) of Kan ' Amp ' colonies and ...
Page 15-108
... region of interest . The major problem of these and other misincorporation methods is the difficulty in creating populations of template molecules in which the 3 ' hydroxyl terminus of the growing strand is located at random positions ...
... region of interest . The major problem of these and other misincorporation methods is the difficulty in creating populations of template molecules in which the 3 ' hydroxyl terminus of the growing strand is located at random positions ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml