Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 80
Page 9-58
... REMOVING PROBES FROM NITROCELLULOSE FILTERS 1. Heat several hundred milliliters of 0.05 × SSC , 0.01 м EDTA ( pH 8.0 ) to boiling . Remove the fluid from the heat and add SDS to a final concen- tration of 0.1 % . Immerse the filter in ...
... REMOVING PROBES FROM NITROCELLULOSE FILTERS 1. Heat several hundred milliliters of 0.05 × SSC , 0.01 м EDTA ( pH 8.0 ) to boiling . Remove the fluid from the heat and add SDS to a final concen- tration of 0.1 % . Immerse the filter in ...
Page 11-36
... remove all of the fluid from the tube , and repeat the washing procedure . 6. Carefully remove all of the fluid from the tube , and add 500 μl of a solution consisting of 80 % ethanol and 20 % 0.1 м sodium acetate ( pH 5.2 ) . Vortex ...
... remove all of the fluid from the tube , and repeat the washing procedure . 6. Carefully remove all of the fluid from the tube , and add 500 μl of a solution consisting of 80 % ethanol and 20 % 0.1 м sodium acetate ( pH 5.2 ) . Vortex ...
Page 13-54
... remove the shark's tooth comb from the top of the gel , and strip the electrical tape from the bottom of the gel mold . This is best done by cutting the tape into several segments with a scalpel blade and then removing each segment in ...
... remove the shark's tooth comb from the top of the gel , and strip the electrical tape from the bottom of the gel mold . This is best done by cutting the tape into several segments with a scalpel blade and then removing each segment in ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml