Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 15-8
... restriction sites . Enzyme ( s ) should be chosen that have potential cleavage sites distributed over the entire length of the target sequence . Often , it is necessary to use two or more restriction enzymes to obtain the desired type ...
... restriction sites . Enzyme ( s ) should be chosen that have potential cleavage sites distributed over the entire length of the target sequence . Often , it is necessary to use two or more restriction enzymes to obtain the desired type ...
Page 15-13
... restriction map of the original plasmid two restriction enzymes that each cleave the plasmid once at different locations . Ideally , these two sites should flank the target DNA . In one tube , digest an aliquot of the test DNA with one ...
... restriction map of the original plasmid two restriction enzymes that each cleave the plasmid once at different locations . Ideally , these two sites should flank the target DNA . In one tube , digest an aliquot of the test DNA with one ...
Page 15-86
... restriction sites within the target DNA . Choose a restriction enzyme to linearize the plasmid that cleaves the target DNA at many different places , preferably generating a protruding terminus two nucleotides in length ( e.g. , TaqI..T ...
... restriction sites within the target DNA . Choose a restriction enzyme to linearize the plasmid that cleaves the target DNA at many different places , preferably generating a protruding terminus two nucleotides in length ( e.g. , TaqI..T ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml