Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 83
Page 9-18
... room temperature , and , if necessary , pour the solution into a centrifuge tube . Add an equal volume of phenol equilibrated with 0.5 M Tris Cl ( pH 8.0 ) ( see Appendix B ) and gently mix the two phases by slowly turning the tube end ...
... room temperature , and , if necessary , pour the solution into a centrifuge tube . Add an equal volume of phenol equilibrated with 0.5 M Tris Cl ( pH 8.0 ) ( see Appendix B ) and gently mix the two phases by slowly turning the tube end ...
Page 9-58
... room temperature . Remove most of the liquid from the filter by placing it on a pad of paper towels . 4. Sandwich the damp filter between two sheets of Saran Wrap , and apply it to X - ray film to check that all of the probe has been ...
... room temperature . Remove most of the liquid from the filter by placing it on a pad of paper towels . 4. Sandwich the damp filter between two sheets of Saran Wrap , and apply it to X - ray film to check that all of the probe has been ...
Page 15-29
... room temperature , and then add approximately 1 μl ( ~ 5 units ) of the Klenow fragment of E. coli DNA polymerase I. Continue the incubation for a further 15 minutes at room temperature . 10 × Polymerase buffer • 200 mM Tris Cl ( pH 7.6 ) ...
... room temperature , and then add approximately 1 μl ( ~ 5 units ) of the Klenow fragment of E. coli DNA polymerase I. Continue the incubation for a further 15 minutes at room temperature . 10 × Polymerase buffer • 200 mM Tris Cl ( pH 7.6 ) ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml