Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 70
Page 13-40
... samples . 6. After all of the samples have been taken , incubate the microfuge tubes or microtiter plate for 30 minutes at 30 ° C . Add 1 μl of S1 stop mixture to each of the microfuge tubes or wells , and incubate for 10 minutes at 70 ...
... samples . 6. After all of the samples have been taken , incubate the microfuge tubes or microtiter plate for 30 minutes at 30 ° C . Add 1 μl of S1 stop mixture to each of the microfuge tubes or wells , and incubate for 10 minutes at 70 ...
Page 13-55
... samples . Excess TBE should be ejected before the next sample is drawn into the syringe and needle . • An automatic micropipettor ( 20 μl ) equipped with a standard tip . Because the tip is too large to fit between the glass plates , the ...
... samples . Excess TBE should be ejected before the next sample is drawn into the syringe and needle . • An automatic micropipettor ( 20 μl ) equipped with a standard tip . Because the tip is too large to fit between the glass plates , the ...
Page 15-28
... samples of the digestion mixture and place in microfuge tubes that contain 2 μl of 0.1 м EDTA ( pH 8.0 ) . Store the collected samples on ice . 7. When all of the samples have been collected , analyze 3 μl of each sample by ...
... samples of the digestion mixture and place in microfuge tubes that contain 2 μl of 0.1 м EDTA ( pH 8.0 ) . Store the collected samples on ice . 7. When all of the samples have been collected , analyze 3 μl of each sample by ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml