Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 54
Page 9-7
... segments of foreign DNA approximately 15-20 kb in length . The genomic DNA libraries constructed in these vectors ... segment of the eukaryotic genome . More significant , however , are the cumulative savings of time and effort during ...
... segments of foreign DNA approximately 15-20 kb in length . The genomic DNA libraries constructed in these vectors ... segment of the eukaryotic genome . More significant , however , are the cumulative savings of time and effort during ...
Page 14-2
... segment of DNA that lies between two regions of known sequence . As shown in Figure 14.1 , two oligonucleotides are used as primers for a series of synthetic reactions that are catalyzed by a DNA polymerase . These oligonucleotides ...
... segment of DNA that lies between two regions of known sequence . As shown in Figure 14.1 , two oligonucleotides are used as primers for a series of synthetic reactions that are catalyzed by a DNA polymerase . These oligonucleotides ...
Page 15-60
... segment of foreign DNA is sequenced in two stages . First , the presence of the desired mutation is confirmed usually by the dideoxy - mediated chain - termination method . In those cases where the target site for mutation lies a ...
... segment of foreign DNA is sequenced in two stages . First , the presence of the desired mutation is confirmed usually by the dideoxy - mediated chain - termination method . In those cases where the target site for mutation lies a ...
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml