Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-42
... separate from it more effectively during electrophoresis . However , even when their lengths are identical , there is a good chance that the template and product strands will separate to some extent during electrophoresis since the rate ...
... separate from it more effectively during electrophoresis . However , even when their lengths are identical , there is a good chance that the template and product strands will separate to some extent during electrophoresis since the rate ...
Page 15-12
... Separate the organic and aqueous phases by cen- trifugation at 12,000g for 3 minutes at 4 ° C in a microfuge . g . Transfer the aqueous phase to a fresh microfuge tube , and add : 10 м ammonium acetate isopropanol 30 μ . 120 μl h ...
... Separate the organic and aqueous phases by cen- trifugation at 12,000g for 3 minutes at 4 ° C in a microfuge . g . Transfer the aqueous phase to a fresh microfuge tube , and add : 10 м ammonium acetate isopropanol 30 μ . 120 μl h ...
Page 15-25
... separate the target DNA from the vector . Incubate the reactions for 1 hour at the appropriate temperature . 13. At the end of the incubation , transfer an aliquot ( 3 μl ) from each digest to a fresh microfuge tube . Store the ...
... separate the target DNA from the vector . Incubate the reactions for 1 hour at the appropriate temperature . 13. At the end of the incubation , transfer an aliquot ( 3 μl ) from each digest to a fresh microfuge tube . Store the ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml