Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 73
Page 8-49
... sequence that have been synthesized in vitro . The sequence of these probes is deduced , using the genetic code , from short regions of the known amino acid sequence of the protein of interest . Because of the degeneracy of the genetic ...
... sequence that have been synthesized in vitro . The sequence of these probes is deduced , using the genetic code , from short regions of the known amino acid sequence of the protein of interest . Because of the degeneracy of the genetic ...
Page 11-4
... sequence and other closely related sequences . Single oligonucleotides of defined sequence are used : • To screen cDNA or genomic DNA libraries , or subclones derived from them , for additional clones containing segments of DNA that ...
... sequence and other closely related sequences . Single oligonucleotides of defined sequence are used : • To screen cDNA or genomic DNA libraries , or subclones derived from them , for additional clones containing segments of DNA that ...
Page 11-5
... sequence of amino acids . For example : ALA TRP LYS Amino acid sequence : VAL ASN PHE TYR A A T T T T T A DNA coding sequences : GT A A TT ΤΑ GC TGG A A C G с C C G G C The sequence within the shaded region contains three codons with ...
... sequence of amino acids . For example : ALA TRP LYS Amino acid sequence : VAL ASN PHE TYR A A T T T T T A DNA coding sequences : GT A A TT ΤΑ GC TGG A A C G с C C G G C The sequence within the shaded region contains three codons with ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml