Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxiii
... Sequencing Reactions 13.61 DIDEOXY - MEDIATED SEQUENCING REACTIONS USING SEQUENASES 13.65 Preparation 13.65 Sequencing Reactions 13.67 SEQUENCING DENATURED DOUBLE - STRANDED DNA TEMPLATES 13.70 Sequencing of Plasmid DNAs Purified by ...
... Sequencing Reactions 13.61 DIDEOXY - MEDIATED SEQUENCING REACTIONS USING SEQUENASES 13.65 Preparation 13.65 Sequencing Reactions 13.67 SEQUENCING DENATURED DOUBLE - STRANDED DNA TEMPLATES 13.70 Sequencing of Plasmid DNAs Purified by ...
Page 13-43
... reactions , in which the annealed oligonucleotide is extended and the newly synthesized chains of DNA are terminated by incorporation of ddATP , ddGTP , ddCTP , or ddTTP . In sequencing reactions using Sequenases , a preliminary step ...
... reactions , in which the annealed oligonucleotide is extended and the newly synthesized chains of DNA are terminated by incorporation of ddATP , ddGTP , ddCTP , or ddTTP . In sequencing reactions using Sequenases , a preliminary step ...
Page 13-76
... sequencing reactions yields four blank tracks All sequencing reactions on gel contain the same blank track Variation in incorporation of radiolabel from template to template Generalized high background in all four lanes makes sequence ...
... sequencing reactions yields four blank tracks All sequencing reactions on gel contain the same blank track Variation in incorporation of radiolabel from template to template Generalized high background in all four lanes makes sequence ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml