Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-18
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single - stranded probes , which consist of only one of the two complementary strands of a given sequence of nucleic acid , have a considerable ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single - stranded probes , which consist of only one of the two complementary strands of a given sequence of nucleic acid , have a considerable ...
Page 14-28
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Protocol II : Sequencing Single - stranded DNA Templates Generated by Asymmetric Amplification In this method ... Single-stranded DNA Templates Generated Asymmetric Amplification 14 28.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Protocol II : Sequencing Single - stranded DNA Templates Generated by Asymmetric Amplification In this method ... Single-stranded DNA Templates Generated Asymmetric Amplification 14 28.
Page 15-20
... double - stranded DNA termini / liter , and ( S ) , = molar concentration of double - stranded termini / liter at t = 0 minutes ( remains essentially constant until a significant portion of the molecules has under- gone complete ...
... double - stranded DNA termini / liter , and ( S ) , = molar concentration of double - stranded termini / liter at t = 0 minutes ( remains essentially constant until a significant portion of the molecules has under- gone complete ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml