Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 81
Page 9-18
... solution . Proteinase K is stored as a stock solution at a concentration of 20 mg / ml in H2O ( see Appendix B ) . 3. Place the suspension of lysed cells in a water bath for 3 hours at 50 ° C . Swirl the viscous solution periodically ...
... solution . Proteinase K is stored as a stock solution at a concentration of 20 mg / ml in H2O ( see Appendix B ) . 3. Place the suspension of lysed cells in a water bath for 3 hours at 50 ° C . Swirl the viscous solution periodically ...
Page 11-50
... solution of TMACI or TEACI through a Whatman No. 1 paper . 4. Filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 - micron pore size ) . Store the filtered solution in dark bottles at room temperature . 5. Measure ...
... solution of TMACI or TEACI through a Whatman No. 1 paper . 4. Filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 - micron pore size ) . Store the filtered solution in dark bottles at room temperature . 5. Measure ...
Page 12-34
... solution , and replace it with fresh solution . Agitate the filters for an additional 5 minutes at 4 ° C . Denaturation solution is made by diluting 10 × binding buffer with five volumes of distilled water . This solution is then added ...
... solution , and replace it with fresh solution . Agitate the filters for an additional 5 minutes at 4 ° C . Denaturation solution is made by diluting 10 × binding buffer with five volumes of distilled water . This solution is then added ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml