Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-14
... specific activity degrade rapidly due to radiochemical decay ( Stent and Fuerst 1960 ) . Probes whose specific activities exceed 2 × 109 cpm / μg must therefore be used without delay . Probes radiolabeled to lower specific activity ...
... specific activity degrade rapidly due to radiochemical decay ( Stent and Fuerst 1960 ) . Probes whose specific activities exceed 2 × 109 cpm / μg must therefore be used without delay . Probes radiolabeled to lower specific activity ...
Page 10-43
... specific activity can be circumvented to a large extent by using subtracted probes radiolabeled to high specific activity . SUBTRACTED PROBES RADIOLABELED TO HIGH SPECIFIC ACTIVITY In this procedure , a preparation of subtracted cDNA is ...
... specific activity can be circumvented to a large extent by using subtracted probes radiolabeled to high specific activity . SUBTRACTED PROBES RADIOLABELED TO HIGH SPECIFIC ACTIVITY In this procedure , a preparation of subtracted cDNA is ...
Page 11-41
... specific activity of the [ a - 32P ] dNTPs in the reaction . The specific activity of a dNTP whose a - phosphate has been completely substituted by 32P is approximately 9000 Ci / mmole . Preparations with lower specific activities ...
... specific activity of the [ a - 32P ] dNTPs in the reaction . The specific activity of a dNTP whose a - phosphate has been completely substituted by 32P is approximately 9000 Ci / mmole . Preparations with lower specific activities ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml