Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 56
Page 9-33
... stored at 4 ° C , it should be heated to 56 ° C for 2-3 minutes before it is applied to the gel . This disrupts any base pairing that may have occurred between protruding cohesive termini . The gel should be run slowly ( < 1 V / cm ) to ...
... stored at 4 ° C , it should be heated to 56 ° C for 2-3 minutes before it is applied to the gel . This disrupts any base pairing that may have occurred between protruding cohesive termini . The gel should be run slowly ( < 1 V / cm ) to ...
Page 9-49
... stored at -20 ° C . The stock solution is diluted tenfold into prehybridization buffer ( usually 6 × SSC or 6 × SSPE containing 0.5 % SDS and 100 μg / ml denatured , fragmented , salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g ...
... stored at -20 ° C . The stock solution is diluted tenfold into prehybridization buffer ( usually 6 × SSC or 6 × SSPE containing 0.5 % SDS and 100 μg / ml denatured , fragmented , salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g ...
Page 10-14
... stored for several days . The average size of the probe is an inverse function of the concentration of primer ( Hodgson and Fisk 1987 ) . The presence of high concentrations of primer is thought to lead to steric hindrance or to ...
... stored for several days . The average size of the probe is an inverse function of the concentration of primer ( Hodgson and Fisk 1987 ) . The presence of high concentrations of primer is thought to lead to steric hindrance or to ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml