Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 88
Page 8-15
The product of the self - primed synthesis of the second strand was a double -
stranded cDNA molecule closed at the terminus corresponding to the 5 ' terminus
of the mRNA by a hairpin loop . This loop was then digested with the single ...
The product of the self - primed synthesis of the second strand was a double -
stranded cDNA molecule closed at the terminus corresponding to the 5 ' terminus
of the mRNA by a hairpin loop . This loop was then digested with the single ...
Page 8-64
Protocol for the Synthesis of the Second Strand of cDNA The unlabeled first -
strand reaction needs no further treatment before proceeding to the synthesis of
the second strand of cDNA . 1 . Add the following reagents directly to the first -
strand ...
Protocol for the Synthesis of the Second Strand of cDNA The unlabeled first -
strand reaction needs no further treatment before proceeding to the synthesis of
the second strand of cDNA . 1 . Add the following reagents directly to the first -
strand ...
Page 8-65
second - strand molecules have been generated by self - priming rather than by
replacement synthesis . This can be verified as follows : a . Treat two aliquots of
radiolabeled first strand and two aliquots of radiolabeled second strand with 1
mM ...
second - strand molecules have been generated by self - priming rather than by
replacement synthesis . This can be verified as follows : a . Treat two aliquots of
radiolabeled first strand and two aliquots of radiolabeled second strand with 1
mM ...
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Contents
CONCENTRATING NUCLEIC ACIDS E | 8-10 |
SYNTHESIS OF THE FIRST STRAND OF DNA 8 | 8-11 |
MEASUREMENT OF RADIOACTIVITY IN NUCLEIC ACIDS E | 8-18 |
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Common terms and phrases
aliquots allow amount amplification antibody appropriate approximately bacteriophage bacteriophage T4 base binding buffer carried cDNA cells centrifugation Chapter chromatography cleaved clones colonies complete concentration constructed containing deletions denatured described detection determine digestion dNTPs double-stranded efficiency electrophoresis et al ethanol expression extraction Figure filters four fragment gene genomic DNA hybridization increase Incubate inserted interest isolated Klenow labeled length libraries ligation linear linkers membrane method minutes mixture molecules mRNA mutants nucleic acids nucleotides obtained oligonucleotide plaques plasmid DNA plate position possible precipitation prepared presence primer probe problem protein purified radioactivity radiolabeled reaction recombinant region remove restriction enzyme room temperature samples screening separate sequence single single-stranded solution step stored strand synthesis target DNA target sequence template termini transfer Tris tube units usually vector volume
Bibliographic information
| Title | Molecular Cloning: A Laboratory Manual, Book 2 Molecular Cloning: A Laboratory Manual, Joseph Sambrook, ISBN 0879693096, 9780879693091 |
| Authors | Joseph Sambrook, E. F. Fritsch, Tom Maniatis |
| Edition | 2 |
| Publisher | Cold Spring Harbor Laboratory, 1989 |
| Original from | the University of Michigan |
| Digitized | 23 Feb 2010 |
| ISBN | 0879693096, 9780879693091 |
| Length | 654 pages |
| Export Citation | BiBTeX EndNote RefMan |