Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 75
Page 11-55
... target . The optimal temperature for hybridization is then determined on the basis of this value . The procedure requires a cloned target sequence that is complementary ( perfectly or imperfectly , depending on the experiment ) to the ...
... target . The optimal temperature for hybridization is then determined on the basis of this value . The procedure requires a cloned target sequence that is complementary ( perfectly or imperfectly , depending on the experiment ) to the ...
Page 14-12
... target sequence . The fragment carrying the target sequence should be no larger than 2-3 kb in length . The DNA is then diluted and ligated under conditions that favor the formation of monomeric circles . Because Taq DNA polymerase ...
... target sequence . The fragment carrying the target sequence should be no larger than 2-3 kb in length . The DNA is then diluted and ligated under conditions that favor the formation of monomeric circles . Because Taq DNA polymerase ...
Page 14-30
... target sequence from the yield of amplified product in a standard polymerase chain reaction . Recently , however , G. Gilliland , S. Perrin , and H.F. Bunn ( pers . comm . ) have devised a method to measure the overall efficiency of ...
... target sequence from the yield of amplified product in a standard polymerase chain reaction . Recently , however , G. Gilliland , S. Perrin , and H.F. Bunn ( pers . comm . ) have devised a method to measure the overall efficiency of ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector