Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-51
... Termini of DNA 5 ' 3 ' The termini of DNA molecules can be radiolabeled enzymatically to yield molecules that may be used in nuclease - S1 mapping of RNA , as radiolabeled primers in primer - extension reactions , and as markers of ...
... Termini of DNA 5 ' 3 ' The termini of DNA molecules can be radiolabeled enzymatically to yield molecules that may be used in nuclease - S1 mapping of RNA , as radiolabeled primers in primer - extension reactions , and as markers of ...
Page 13-78
... termini of the DNA . Asymmetric Labeling of Target DNA Double - stranded DNA can be labeled in the following ways : • At its 5 ' termini by bacteriophage T4 polynucleotide kinase and [ y - 32P ] ATP ( see Chapter 11 , pages 11.31-11.32 ) ...
... termini of the DNA . Asymmetric Labeling of Target DNA Double - stranded DNA can be labeled in the following ways : • At its 5 ' termini by bacteriophage T4 polynucleotide kinase and [ y - 32P ] ATP ( see Chapter 11 , pages 11.31-11.32 ) ...
Page 15-20
... termini / liter , and ( S ) , = molar concentration of double - stranded termini / liter at t = 0 minutes ( remains essentially constant until a significant portion of the molecules has under- gone complete degradation ) . At an enzyme ...
... termini / liter , and ( S ) , = molar concentration of double - stranded termini / liter at t = 0 minutes ( remains essentially constant until a significant portion of the molecules has under- gone complete degradation ) . At an enzyme ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector