Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 38
Page 10-27
... TRANSCRIPTION OF DOUBLE - STRANDED DNA TEMPLATES BY BACTERIOPHAGE DNA- DEPENDENT RNA POLYMERASES The technical advance that made it possible to synthesize single - stranded RNA probes of high specific activity was the development of ...
... TRANSCRIPTION OF DOUBLE - STRANDED DNA TEMPLATES BY BACTERIOPHAGE DNA- DEPENDENT RNA POLYMERASES The technical advance that made it possible to synthesize single - stranded RNA probes of high specific activity was the development of ...
Page 10-32
... transcription of linearized plasmid DNA contain- ing a bacteriophage promoter are : 40 mM Tris Cl ( pH 7.5 ) 6 mM MgCl2 2 mm spermidine HCl 5 mM NaCI 10 mm dithiothreitol 100 μg / ml bovine serum albumin ( Fraction V ; Sigma ) 20 nm ...
... transcription of linearized plasmid DNA contain- ing a bacteriophage promoter are : 40 mM Tris Cl ( pH 7.5 ) 6 mM MgCl2 2 mm spermidine HCl 5 mM NaCI 10 mm dithiothreitol 100 μg / ml bovine serum albumin ( Fraction V ; Sigma ) 20 nm ...
Page 10-36
... transcription systems include : • No RNA synthesis . The most common cause of an apparent lack of RNA synthesis is contamination of tubes or reagents with RNAase . This can be avoided by taking the precautions described in Chapter 7 ...
... transcription systems include : • No RNA synthesis . The most common cause of an apparent lack of RNA synthesis is contamination of tubes or reagents with RNAase . This can be avoided by taking the precautions described in Chapter 7 ...
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml