Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 17
Page xi
... Transfection of Competent Bacteria 4.33 Cloning DNA Fragments into Bacteriophage M13 Vectors 4.35 Preparation of Competent Bacteria 4.36 Transfection of Competent Bacteria with Bacteriophage M13 DNA 4.37 Identification and Analysis of ...
... Transfection of Competent Bacteria 4.33 Cloning DNA Fragments into Bacteriophage M13 Vectors 4.35 Preparation of Competent Bacteria 4.36 Transfection of Competent Bacteria with Bacteriophage M13 DNA 4.37 Identification and Analysis of ...
Page xxvi
... Transfection of Cells Growing in Suspension 16.38 Modified Calcium Phosphate - mediated Transfection Procedure 16.39 TRANSFECTION MEDIATED BY DEAE - DEXTRAN 16.41 Transfection Using DEAE - Dextran : Protocol I 16.42 Transfection Using ...
... Transfection of Cells Growing in Suspension 16.38 Modified Calcium Phosphate - mediated Transfection Procedure 16.39 TRANSFECTION MEDIATED BY DEAE - DEXTRAN 16.41 Transfection Using DEAE - Dextran : Protocol I 16.42 Transfection Using ...
Page 15-59
... transfection . The aim of this step was to reduce the background of plaques containing wild - type target DNA . However , this time - consuming enrichment procedure is no longer necessary because of the improvement in efficiency of ...
... transfection . The aim of this step was to reduce the background of plaques containing wild - type target DNA . However , this time - consuming enrichment procedure is no longer necessary because of the improvement in efficiency of ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml