Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-34
... transfer fragments of DNA from agarose gels to solid supports ( nitrocellulose filters or nylon membranes ) : 1. Capillary transfer . In the capillary transfer method ( Southern 1975 ) , DNA fragments are carried from the gel in a flow ...
... transfer fragments of DNA from agarose gels to solid supports ( nitrocellulose filters or nylon membranes ) : 1. Capillary transfer . In the capillary transfer method ( Southern 1975 ) , DNA fragments are carried from the gel in a flow ...
Page 9-36
... transfer is generally complete within 2-3 hours . Because elec- trophoretic transfer requires comparatively large electric currents , it is often difficult to maintain the electrophoresis buffer at a temperature compatible with ...
... transfer is generally complete within 2-3 hours . Because elec- trophoretic transfer requires comparatively large electric currents , it is often difficult to maintain the electrophoresis buffer at a temperature compatible with ...
Page 9-38
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Transfer of DNA to Nitrocellulose Filters CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 1. After electrophoresis , transfer the gel to a glass baking dish and trim away any unused ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Transfer of DNA to Nitrocellulose Filters CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 1. After electrophoresis , transfer the gel to a glass baking dish and trim away any unused ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector