Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-5
... transformants that have taken up and stably maintained an artificial chromosome are identified as colonies on agar plates containing the components necessary for selection of one or both YAC arms . YAC vectors are designed to allow ...
... transformants that have taken up and stably maintained an artificial chromosome are identified as colonies on agar plates containing the components necessary for selection of one or both YAC arms . YAC vectors are designed to allow ...
Page 15-13
... transformants , estimate the number of independent transformants that will be required to saturate the original blunt - ended restriction sites in the target DNA with the desired density of linkers . 9. After preliminary restriction ...
... transformants , estimate the number of independent transformants that will be required to saturate the original blunt - ended restriction sites in the target DNA with the desired density of linkers . 9. After preliminary restriction ...
Page 15-44
... transformants should contain both kanamycin and ampicillin , each at a concentration of 100 μg / ml ) . Store the remainders of the ligation mixtures at -20 ° C until step 2 of the protocol has been completed . For definition of Weiss ...
... transformants should contain both kanamycin and ampicillin , each at a concentration of 100 μg / ml ) . Store the remainders of the ligation mixtures at -20 ° C until step 2 of the protocol has been completed . For definition of Weiss ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml