Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-56
... units ( Weiss et al . 1968 ) . One Weiss unit is the amount of enzyme that catalyzes the exchange of 1 nmole of 32P from pyrophos- phate into [ y , ẞ - 32P ] ATP in 20 minutes at 37 ° C . One Weiss unit corre- sponds to 0.2 unit ...
... units ( Weiss et al . 1968 ) . One Weiss unit is the amount of enzyme that catalyzes the exchange of 1 nmole of 32P from pyrophos- phate into [ y , ẞ - 32P ] ATP in 20 minutes at 37 ° C . One Weiss unit corre- sponds to 0.2 unit ...
Page 12-32
... units ( Weiss et al . 1968 ) . One Weiss unit is the amount of enzyme that catalyzes the exchange of 1 nmole of 32P from pyrophosphate into [ y , B - 32P ] ATP in 20 minutes at 37 ° C . One Weiss unit corresponds to 0.2 unit determined ...
... units ( Weiss et al . 1968 ) . One Weiss unit is the amount of enzyme that catalyzes the exchange of 1 nmole of 32P from pyrophosphate into [ y , B - 32P ] ATP in 20 minutes at 37 ° C . One Weiss unit corresponds to 0.2 unit determined ...
Page 15-30
... units ( Weiss et al . 1968 ) . One Weiss unit is the amount of enzyme that catalyzes the exchange of 1 nmole of 32P from pyrophosphate into [ y , B - 32P ] ATP in 20 minutes at 37 ° C . One Weiss unit corresponds to 0.2 unit determined ...
... units ( Weiss et al . 1968 ) . One Weiss unit is the amount of enzyme that catalyzes the exchange of 1 nmole of 32P from pyrophosphate into [ y , B - 32P ] ATP in 20 minutes at 37 ° C . One Weiss unit corresponds to 0.2 unit determined ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml