Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 83
Page 8-47
... Usually , a fragment derived from one end or the other of the existing clone is isolated , radiolabeled in vitro , and used to probe a library . Hybridization with homologous probes is always carried out under stringent conditions ...
... Usually , a fragment derived from one end or the other of the existing clone is isolated , radiolabeled in vitro , and used to probe a library . Hybridization with homologous probes is always carried out under stringent conditions ...
Page 11-48
... Usually , conditions are chosen to be 2 ° C below the calculated Tm of the most A / T - rich member of the pool ( Suggs et al . 1981b ) . However , the use of such " lowest common denominator " conditions can lead to a number of false ...
... Usually , conditions are chosen to be 2 ° C below the calculated Tm of the most A / T - rich member of the pool ( Suggs et al . 1981b ) . However , the use of such " lowest common denominator " conditions can lead to a number of false ...
Page 14-15
... ( usually 72 ° C ) . It is assumed that the Taq DNA polymer- ase begins to work , albeit sluggishly , as soon as the ... Usually , oligonucleotides are used at a concentration of 1 μм in polymerase chain reactions . This is usually ...
... ( usually 72 ° C ) . It is assumed that the Taq DNA polymer- ase begins to work , albeit sluggishly , as soon as the ... Usually , oligonucleotides are used at a concentration of 1 μм in polymerase chain reactions . This is usually ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector